Shuang Hou

Mar 7, 2024 | nature.com | Shuang Hou |Dan Li |Cong Liu

AbstractThe burgeoning comprehension of protein phase separation (PS) has ushered in a wealth of bioinformatics tools for the prediction of phase-separating proteins (PSPs). These tools often skew towards PSPs with a high content of intrinsically disordered regions (IDRs), thus frequently undervaluing potential PSPs without IDRs. Nonetheless, PS is not only steered by IDRs but also by the structured modular domains and interactions that aren’t necessarily reflected in amino acid sequences.

Sep 6, 2023 | mdpi.com | Yan Du |Shuang Hou |Zhou Chen |Wancheng Li

2. Materials and Methods 2.1. Data Downloading and ProcessingTo date, The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) contain a total of 178 PAAD tissues and 171 healthy pancreatic tissues. The University of California Santa Cruz (UCSC) dataset compiles and normalizes high-throughput sequencing (HTSeq) data from TCGA and GTEx, which can be utilized to conduct differential expression analyses of genes [21].