Sidney Y Vermeulen

Apr 28, 2024 | biorxiv.org | Richard Hall |Hongquan Li |Sidney Y Vermeulen |Robin A Rio

AbstractLive imaging of regenerative processes can reveal how animals restore their bodies after injury through a cascade of dynamic cellular events. Here, we present a comprehensive toolkit for live imaging of whole-body regeneration in the flatworm Macrostomum lignano, including a high throughput cloning pipeline, targeted cellular ablation, and advanced microscopy solutions. Using tissue-specific reporter expression, we examine how various structures regenerate.