Sophie E. Clough

2 months ago | biorxiv.org | Sophie E. Clough |Arthur Glasfeld |Nigel Robinson

AbstractHere we provide the background data supporting an additional web-based calculator to predict in-cell metalation of proteins within E. coli BW25113 grown in M9 media. Intracellular metal availabilities have been estimated from the calibrated responses of DNA-binding, metal-sensing, transcriptional regulators. We have previously tested and validated metalation calculator predictions. They confirm that when proteins are expressed heterologously in E.