Sucharita Sarkar

3 weeks ago | cell.com | Sucharita Sarkar |Ian MacRae |Ian Macrae

Get full text accessLog in, subscribe or purchase for full access. References1. Bobadilla Ugarte, P. ∙ Halter, S. ∙ Mutte, S.K. ... Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNAMol. Cell. 2025; 85:1920-1937.e102. Bernstein, E. ∙ Caudy, A.A. ∙ Hammond, S.M. ... Role for a bidentate ribonuclease in the initiation step of RNA interferenceNature. 2001; 409:363-3663. Zamore, P.D. ∙ Tuschl, T. ∙ Sharp, P.A. ...

Dec 6, 2024 | biorxiv.org | Sucharita Sarkar |Ian MacRae |Ian Macrae

AbstractSmall interfering RNAs (siRNAs) guide mRNA cleavage by human Argonaute2 (hAgo2), leading to targeted gene silencing. Despite their laboratory and clinical impact, structural insights into human siRNA catalytic activity remain elusive. Here, we show that disrupting siRNA 3'end binding by hAgo2 accelerates target cleavage and stabilizes its catalytic conformation, enabling detailed structural analysis.