Takashi Sumikama

1 month ago | biorxiv.org | Kien Ngo |Takashi Sumikama |Remi Vuillemot |Han Nguyen

AbstractAlpha-actinin is a crucial actin-binding protein involved in cellular functions like muscle contractility, mechanosensation, and cell division. Studying its atomic structure and dynamics is challenging due to protein complexity and imaging limitations. To address this, we developed SimHS-AFMfit, integrating high-speed AFM (HS-AFM), AFMfit (a flexible fitting technique), and molecular dynamics (MD) simulations.