Thibault Colombani

Jun 14, 2024 | onlinelibrary.wiley.com | Zachary Rogers |Thibault Colombani |Saad Khan |Khushbu Bhatt

Bulk Proteomic Sample Preparation Cells were removed from O2-controlled incubators, immediately placed on ice, centrifuged at 4 °C, resuspended in Mass Spectrometry grade Water (Fisher Scientific, W6500), and then frozen at −80 °C. Cells were lysed by heating at 90 °C for 10 min,[89] and protein concentrations for each lysate were measured using a Nanodrop (A205).