Virginia Apostolopoulou

Nov 8, 2024 | biorxiv.org | Alisia Fadini |Thomas Lane |Deutsches Elektronen-Synchrotron DESY |Virginia Apostolopoulou

AbstractAdvances in structural biology increasingly focus on uncovering protein dynamics and transient or weak macromolecular complexes. Such studies require modeling of low-occupancy species, for instance time-evolving intermediates and bound ligands. In protein crystallography, difference maps that compare paired perturbed and reference datasets are a powerful way to identify and aid modeling of low-occupancy species.