Xiaolong Wu

Jan 12, 2025 | mdpi.com | Xiaolong Wu |Susu Yang |Jun Li |Jian Qiu

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Dec 26, 2024 | mdpi.com | Xiaolong Wu

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Jul 28, 2023 | dx.doi.org | Zhiwen Xi |Lihong Li |zhiyong Liu |Xiaolong Wu

l-threo-p-methylsulfonylphenylserine (compound 1b) is the main intermediate of florfenicol, and its efficient synthesis has been the subject of current research. Herein, Burkholderia diffusa l-threonine transaldolase (BuLTTA) was rationally designed based on the sequence–structure–function relationship. A mutant M4 (Asn35Ser/Thr352Asn) could produce 35.5 mM 1b with 88.8% conversion and 93.8% diastereoselectivity, 314 and 129% of the values observed for wild-type BuLTTA.

Jul 28, 2023 | pubs.acs.org | Zhiwen Xi |Lihong Li |zhiyong Liu |Xiaolong Wu

Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Agric. Food Chem. 2023, XXXX, XXXRETURN TO ARTICLES ASAPBiotechnology and Bi...Biotechnology and Biological TransformationsNEXTZhiwen XiLab of Brewing Microbiology and Applied Enzymology, School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, P. R.