Yinwen Huo

Feb 15, 2024 | opg.optica.org | Wei Zheng |Jia Yu |Hui Li |Yinwen Huo

Compared to intensity detection, fluorescence lifetime has the advantage of being unaffected by variations in excitation intensity, fluorophore concentration, or attenuation due to biological absorption and scattering. In this Letter, to the best of our knowledge, we present the use of the two-photon excitation autofluorescence lifetime imaging of tryptophan (TRP) to probe cell metabolism for the first time.