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Chromatographyonline.com stands out as the leading global hub for objective, peer-reviewed technical insights into chromatography and separation sciences. We integrate content from our regional publications (LCGC North America, LCGC Europe, and LCGC Asia-Pacific) to provide hands-on, practical information. Our aim is to support scientists and lab managers in mastering chromatographic methods and tools, ultimately enhancing laboratory efficiency and contributing to the success of businesses worldwide.

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Merck Launches 2025 Global Curiosity Cube Tour to Expand STEM Education Access

1 week ago | chromatographyonline.com | Kate Jones

Merck (Darmstadt, Germany) has launched its 2025 Curiosity Cube tour, aiming to bring hands-on STEM (science, technology, engineering, and mathematics) experiences to students across Europe, North America, and, for the first time, Africa (1). The initiative seeks to reach 60,000 students across the world throughout the year. The Curiosity Cube is a solar-powered mobile science lab designed to engage students with interactive experiments and lessons.
A Novel LC-QTOF-MS DIA Method for Pesticide Quantification and Screening in Agricultural Waters

1 week ago | chromatographyonline.com | Kate Jones

As agricultural practices continue to apply pesticides at scale, evaluating their presence—and that of their transformation products—in surface water (SW) and groundwater (GW) remains a key environmental monitoring challenge.
Paul Ferguson Named Professor by Special Appointment at the University of Amsterdam

1 week ago | chromatographyonline.com | Kate Jones

LCGC International Editorial Advisory Board (EAB) member Paul Ferguson has been named a professor by special appointment at the Faculty of Science at the University of Amsterdam, The Netherlands, according to a press release (1). His new position, focused on "Separation of Biomacromolecules with a Special Emphasis on Sustainable Analytical Science," is housed within the Van 't Hoff Institute for Molecular Sciences (HIMS) in Amsterdam, The Netherlands, and is supported by the Bèta Plus foundation.
Navigating The Path To Modern Slalom Chromatography: An Interview with Fabrice Gritti

2 weeks ago | chromatographyonline.com | Alasdair Matheson

Can you define slalom chromatography (SC)? The definition of SC is historical. The term "slalom chromatography" was first introduced, somewhat arbitrarily, as a new mode and mechanism of retention/separation in liquid chromatography in a 1989 publication: Size-Dependent Chromatographic Separation of Double-Stranded DNA Which Is Not Based on Gel Permeation Mode (Analytical Biochemistry, 178, 336–341). (1).
Slalom Chromatography Revisited (Part 1): An Interview With Fabrice Gritti

2 weeks ago | chromatographyonline.com | Alasdair Matheson

LCGC International spoke to Fabrice Gritti, consultant scientist at Waters Corporation, about the history of slalom chromatography (SC) and why he decided that the technique was worth re-investigating. The potential benefits of SC, according to Gritti, include identifying RNA impurities when manufacturing mRNA therapeutics, and assisting in the development of new gene and cell therapies, and other biopharmaceutical applications.
Identifying TB Markers Using UHPLC-IMS-HRMS and Non-Invasive Skin Sampling

2 weeks ago | chromatographyonline.com | Kate Jones

A team of scientists from the University of Pretoria in South Africa, developed a non-invasive approach utilizing ultrahigh-performance liquid chromatography–ion mobility spectrometry-high-resolution mass spectrometry (UHPLC–IMS-HRMS) for the identification of biological markers for tuberculosis (TB) diagnosis using human skin (1). Their findings were published in the Journal of Chromatography Open.
Unravelling Indian Cobra Venom Using 2D-LC–MS

3 weeks ago | chromatographyonline.com | Kate Jones

Researchers at the Indian Institute of Technology Delhi in New Delhi, India, introduced a two-dimensional liquid chromatography–mass spectrometry (2D-LC–MS) platform for the separation of proteins from the venom of the Indian cobra snake (Naja naja). Published in the Journal of Chromatography Open, the work presents an online 2D workflow that combines hydrophobic interaction chromatography (HIC) in the first dimension and reversed-phase LC (RPLC) in the second (1).
A Sustainable Approach to Green Sample Preparation and Analytical Performance Using HS-SPME-GC-QTOF-MS

3 weeks ago | chromatographyonline.com | Kate Jones

April 23, 2025LCGC International spoke to Adrián de la Fuente and Silvia Valverde Bastardo from the University of Valladolid, Spain, about the development of a miniaturized headspace solid-phase microextraction gas chromatography–quadrupole time-of-flight mass spectrometry (HS-SPME-GC–QTOF-MS) method for the analysis of biogenic volatile organic compounds (BVOCs) emitted by native Spanish trees.
How Green is the Future of Chromatography?

4 weeks ago | chromatographyonline.com | Kate Jones

As the world becomes ever more conscious of environmental sustainability, science continues to evolve to meet the growing demand for greener practices. With its widespread application in pharmaceuticals, environmental monitoring, food safety, and forensics, chromatography has a role to play in reducing the environmental footprint of the laboratory.
Understanding FDA Recommendations for N-Nitrosamine Impurity Levels

4 weeks ago | chromatographyonline.com | Susan Haigney

Nitrosamines emerged as a growing concern in pharmaceuticals in 2018, prompting regulatory agencies to collaborate with drug manufacturers to reduce the presence of these potentially cancer-causing compounds in medications. (1).