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2 weeks ago | 
chromatographyonline.com | Alasdair Matheson
Can you define slalom chromatography (SC)? The definition of SC is historical. The term "slalom chromatography" was first introduced, somewhat arbitrarily, as a new mode and mechanism of retention/separation in liquid chromatography in a 1989 publication: Size-Dependent Chromatographic Separation of Double-Stranded DNA Which Is Not Based on Gel Permeation Mode (Analytical Biochemistry, 178, 336–341). (1).
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2 weeks ago | 
chromatographyonline.com | Alasdair Matheson
LCGC International spoke to Fabrice Gritti, consultant scientist at Waters Corporation, about the history of slalom chromatography (SC) and why he decided that the technique was worth re-investigating. The potential benefits of SC, according to Gritti, include identifying RNA impurities when manufacturing mRNA therapeutics, and assisting in the development of new gene and cell therapies, and other biopharmaceutical applications.
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4 weeks ago | 
chromatographyonline.com | Alasdair Matheson
Chromatography plays a crucial role in the research and analysis of per- and polyfluoroalkyl substances (PFAS), a group of synthetic chemicals that have raised significant environmental and health concerns. Due to their unique chemical structure, PFAS are highly persistent in both the environment and living organisms, leading to their widespread presence in water, soil, and food (1).
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
Scientific research into the impact of per- and polyfluoroalkyl substances (PFAS) on human health and the environment is hindered by limited access to high-purity reference standards, according to a team of researchers led by Xenia Trier, an associate professor from the University of Copenhagen, Denmark, and Stefan van Leeuwen, a senior scientist focusing on chemical pollutants at Wageningen University and Research, The Netherlands (1).
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
You recently published a manuscript paper entitled “Implementation of Matrix-Matched Semiquantification of PFAS in AFFF-Contaminated Soil” (1). What is matrix-matched semiquantification and why is semiquantification necessary? Semiquantification is used to estimate the concentration of chemical substances, such as per- and polyfluoroalkyl substances (PFAS), if analytical reference standards are not available.
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
Can you describe the chromatographic and sample preparation techniques selected for your recent research project investigating variations in concentrations of per- and polyfluoroalkyl substances in estuaries in relation to tidal cycles (1)? Did you encounter any challenges from an analytical chemistry perspective, and if so, how did you overcome them? Navneet (Nav) Singh: Solid-phase extraction (SPE) was the primary sample preparation method for separating PFAS.
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
LCGC International spoke to Lauren Lovejoy, principal scientist in the Drug Substance and Product Analysis group at GSK (Philadelphia, Pennsylvania), about the advantages of using tandem columns in sequential elution liquid chromatography (SE-LC) to investigate weak acids, neutral compounds, and permanent anions in pharmaceutical applications. Q: What is sequential elution liquid chromatography (SE-LC), and how does it differ from conventional high performance liquid chromatography (HPLC)?
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
You recently published a manuscript analyzing per- and polyfluoroalkyl substances (PFAS) contamination in agricultural soils (1). This involved exploring the non-targeted identification of precursors, fluorine mass balance, and microcosm studies. What was the rationale behind this research, and can you clarify the three main components of this study: identification of precursors, fluorine mass balance, and microcosm studies?
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
Liquid chromatography–mass spectrometry (LC–MS) and LC–MS/MS techniques are commonly used for per- and polyfluoroalkyl substances (PFAS) analysis. Why did you decide to explore headspace solid-phase microextraction (HS-SPME) and direct immersion SPME (DI-SPME) for this application using gas chromatography (GC–MS) (1)?
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1 month ago | 
chromatographyonline.com | Alasdair Matheson
LCGC International spoke with Daniel Meston and Dwight Stoll from Gustavus Adolphus College in St. Peter, Minnesota, USA, about a project they worked on with Todd Maloney from Eli Lilly in Indianapolis, Indiana, USA, to investigate the optimal performance conditions of antisense oligonucleotides (ASOs) when using hydrophilic interaction liquid chromatography (HILIC). Why are antisense oligonucleotides (ASOs) important therapeutic molecules and what treatments are they used for?
