Abigail L Colclough

Aug 1, 2024 | biorxiv.org | Hannah Pugh |Elizabeth Darby |Leah J. Burgess |Abigail L Colclough

AbstractTripartite resistance-nodulation-division (RND) efflux pumps confer multidrug resistance (MDR) in Gram-negative bacteria and are critical for many physiological functions including virulence and biofilm formation. The common laboratory strain of E. coli, K-12 MG1655 has six recognised RND transporters participating in tripartite pump formation (AcrB, AcrD, AcrF, CusA, MdtBC, and MdtF). However, by studying >20,000 E. coli genomes we show that E.