Achanta Ravi Chandra

Feb 21, 2023 | cell.com | Friedrich-Alexander-Universität Erlangen-Nürnberg |Thomas Gruber |Efthymios Oikonomou |Achanta Ravi Chandra

Single-channel patch-clamp recordings allow observing the action of a single protein complex in real time and hence the deduction of the underlying conformational changes in the ion channel protein. Commonly, recordings are modeled using hidden Markov chains, connecting open and closed states in the experimental data with protein conformations. The rates between states denote transition probabilities that could be modified by membrane voltage or ligand binding.