Alejandro Diaz-Moscoso

2 months ago | nature.com | Ismael Moreno-Sanchez |Pedro Martínez-García |Laura Tomás-Gallardo |Alejandro Diaz-Moscoso |Manuel Munoz |Manuel Muñoz | +5 more

AbstractCRISPR-Cas13 RNA-targeting systems are widely used in basic and applied sciences. However, its application has recently generated controversy due to collateral activity in mammalian cells and mouse models. Moreover, its competence could be improved in vivo. Here, we optimized transient formulations as ribonucleoprotein complexes or mRNA-gRNA combinations to enhance the CRISPR-RfxCas13d system in zebrafish.