Alexandra Langford-Avelar

Sep 24, 2023 | biorxiv.org | Lisa-Marie Legault |Mélanie Breton-Larrivée |Alexandra Langford-Avelar |Anthony Lemieux

AbstractBackground: The placenta is vital for fetal development and its contributions to various developmental issues, such as pregnancy complications, fetal growth restriction, and maternal exposure, have been extensively studied in mice. Contrary to popular belief, the placenta forms mainly from fetal tissue; therefore, it has the same biological sex as the fetus it supports.

Mar 1, 2023 | faseb.onlinelibrary.wiley.com | Elizabeth Elder |Lisa-Marie Legault |Alexandra Langford-Avelar |Mélanie Breton-Larrivée

2.6 Bioinformatics analysis of DNA methylation Quantification analysis of ICR DNA methylation levels was based on the Implicon bioinformatics pipeline.127 First, the eight-random nucleotide sequences serving as UMIs were filtered out and inserted into the readID of the two sequencing reads for deduplication afterward. Next, the lower quality sequences and the adaptor sequences were filtered out of the raw sequencing reads. These two steps were accomplished with Trim Galore (version 0.6.6).