Alfredo Bellisario

Jul 5, 2024 | nature.com | Leonardo Monrroy |Alfredo Bellisario |Richard Bean |Johan Bielecki |Richard A. Kirian |Romain Letrun | +6 more

AbstractDetecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain.