Annapurna Poduri

Jan 16, 2025 | biorxiv.org | Christopher McGraw |Cristina Baker |Annapurna Poduri

AbstractZebrafish models of genetic epilepsy benefit from the ability to assess disease-relevant knock-out alleles with numerous tools, including genetically encoded calcium indicators (GECIs) and hypopigmentation alleles to improve visualization. However, there may be unintended effects of these manipulations on the phenotypes under investigation. There is also debate regarding the use of stable loss-of-function (LoF) alleles in zebrafish, due to genetic compensation (GC).

Aug 5, 2024 | biorxiv.org | Christopher McGraw |Annapurna Poduri

AbstractIdentifying new, more efficacious anti-seizure medications (ASMs) is challenging, partly due to limitations in animal-based assays. Zebrafish (Danio rerio) can serve as a model of chemical and genetic seizures, but methods for detecting seizure-like activity in zebrafish, though powerful, have been hampered by low sensitivity (locomotor/behavioral assays) or low-throughput (tectal electrophysiology or calcium fluorescence microscopy).

Oct 23, 2023 | nature.com | Adithe C. Rivaldi |Noriyuki Shinagawa |Sahib Sran |Mark Hester |Mohammad Marhabaie |Manish Shah | +4 more

AbstractSomatic mosaicism is a known cause of neurological disorders, including developmental brain malformations and epilepsy. Brain mosaicism is traditionally attributed to post-zygotic genetic alterations arising in fetal development. Here we describe post-zygotic rescue of meiotic errors as an alternate origin of brain mosaicism in patients with focal epilepsy who have mosaic chromosome 1q copy number gains.