Benjamin Atha

1 week ago | biocompare.com | Benjamin Atha

Cancer continues to be the leading cause of death worldwide, and its widespread impact means that most people have either faced it themselves or supported someone who has.1 Although our understanding of the many diseases classified as cancer has advanced, there are still many unknowns. One of the most powerful tools driving cancer research forward is next-generation sequencing (NGS).

2 weeks ago | biocompare.com | Benjamin Atha

Thanks to a range of advanced methods, quantifying nucleic acids has become more accessible than ever. Still, challenges persist when working with sample types that are difficult to measure accurately. While researchers aim for consistent, easily quantifiable inputs, some materials are inherently problematic and often unavoidable in molecular studies. Because most lab workflows require precise amounts of DNA or RNA for reliable results, selecting the right quantification method is essential.

Jul 10, 2024 | biocompare.com | Benjamin Atha

Digital PCR (dPCR) is an advanced approach for the absolute quantification of specific DNA and RNA sequences by using partitioned amplification reactions. This method is an adaptation of traditional PCR techniques, offering improved precision, sensitivity, and accuracy. Some advantages of dPCR over other quantification methods include independence from standard curves, higher tolerance to PCR inhibitors, and the capability to detect low-abundance targets.

May 27, 2024 | biocompare.com | Benjamin Atha

Single-cell sequencing represents a major advancement in genomics, offering researchers the ability to discern cellular heterogeneity at unprecedented resolution. For effective single-cell experiments, proper sample isolation and nuclei counting are required. Automated cell counters have become essential components of this workflow, providing reliable quantification across diverse sample types.