Birte Svensson

Sep 11, 2023 | pubs.acs.org | Yu Wang |Birte Svensson |Bernard Henrissat |Marie Sofie Møller

Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Agric. Food Chem. 2023, XXXX, XXXRETURN TO ARTICLES ASAPBiotechnology and Bi...Biotechnology and Biological TransformationsNEXTYu WangEnzyme and Protein Chemistry, Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark, Birte SvenssonEnzyme and Protein Chemistry, Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs.

Sep 11, 2023 | dx.doi.org | Yu Wang |Birte Svensson |Bernard Henrissat |Marie Sofie Møller

Pullulanases are multidomain α-glucan debranching enzymes with one or more N-terminal domains (NTDs) including carbohydrate-binding modules (CBMs) and domains of unknown function (DUFs). To elucidate the roles of NTDs in Lactobacillus acidophilus NCFM pullulanase (LaPul), two truncated variants, Δ41-LaPul (lacking CBM41) and Δ(41+DUFs)-LaPul (lacking CBM41 and two DUFs), were produced recombinantly.