Brett J.G. Estes

Oct 29, 2024 | biorxiv.org | Nisha Gandhi |Jessica Von Stetina |Dev Paudel |Brett J.G. Estes

AbstractRecent advancements in gene insertion have shifted from DNA repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo to insert transgenes in a targeted, unidirectional manner. In vivo, where nuclear delivery of DNA is challenging, adeno-associated virus (AAV) can act as the cargo vector.