Jessica Von Stetina

Nov 3, 2024 | biorxiv.org | Maike Thamsen Dunyak |Patrick Hanna |Angela X Nan |Jessica Von Stetina

AbstractIntegrase-mediated Programmable Genomic Integration (I-PGI) uses a Cas9 nickase (nCas9) with a reverse transcriptase (RT), to write a large serine integrase (LSI) target site (attB/P, here called beacon) in a programmed location. Co-delivery of the LSI and a DNA template containing the cognate recognition site results in precise integration of the template in a specific genomic location.

Oct 29, 2024 | biorxiv.org | Nisha Gandhi |Jessica Von Stetina |Dev Paudel |Brett J.G. Estes

AbstractRecent advancements in gene insertion have shifted from DNA repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo to insert transgenes in a targeted, unidirectional manner. In vivo, where nuclear delivery of DNA is challenging, adeno-associated virus (AAV) can act as the cargo vector.

Oct 21, 2024 | biorxiv.org | Joshua Rose |Hyaeyeong Kim |Jessica Von Stetina |Mahant Thangavelu

AbstractMany current genome editing technologies rely on the action of large serine integrases (LSIs) to insert gene-sized DNA sequences into the genome. Bxb1 is the most commonly used LSI for therapeutic efforts, including PASTE, PASSIGE and I-PGI. While Bxb1 demonstrated good activity in vitro in cycling cells, the activity in non-dividing hepatocytes was significantly less efficient.

Aug 23, 2023 | biorxiv.org | Gustavo C. Cerqueira |Jaime Prout |Jessica Von Stetina |Madison Chasse

AbstractAdeno-associated viral (AAV) vectors are used to treat genetic diseases, expressing therapeutic genes from both extrachromosomal episomes and payloads that integrate into the host genome. Assays were developed to evaluate HR-mediated on-target integration and the potential occurrence of off-target integration.