Bronwyn A Lucas

Oct 3, 2024 | biorxiv.org | Kexin Zhang |Pilar Cossio |Aaditya V Rangan |Bronwyn A Lucas

Abstract2D template matching (2DTM) can be used to detect molecules and their assemblies in cellular cryo-EM images with high positional and orientational accuracy. While 2DTM successfully detects spherical targets such as large ribosomal subunits, challenges remain in detecting smaller and more aspherical targets in various environments. In this work, a novel 2DTM metric, referred to as the 2DTM p-value, is developed to extend the 2DTM framework to more complex applications.