Byrd AL

Oct 23, 2024 | embopress.org | Byrd AL

IntroductionAdvancements in synthetic and systems biology have opened new avenues for utilizing microorganisms as live biotherapeutic products (LBP) (FDA, 2016). The appeal of bacteria as LBP lies in their ability to colonize specific human tissues and adapt to the environment to continuously produce and deliver multiple therapeutic agents locally. This reduces dosage requirements, unspecific adverse effects related to systemic administration and production costs.

May 12, 2024 | ccforum.biomedcentral.com | Byrd AL

A prospective, single-blinded, randomized controlled trial design was used. Between September 2019 and December 2023, patients who were admitted to intensive care units (ICUs) and met the inclusion criteria were randomly assigned to the experimental group or control group. Randomization was performed through a block randomization scheme with a block size of 4, and carried out by an independent statistician.

Dec 19, 2023 | bmcgenomics.biomedcentral.com | Byrd AL

IRB approval was obtained for the study. 19 participants were recruited and consented, and four total collections of four tissue types were completed. The first collection occurred 6 months prior to confirm piloted procedures were ready to scale, and the remaining 3 collections were performed within a 2–4 week window per participant. 75 samples of each tissue type were obtained (1 participant provided only 3 collections).

Jun 30, 2023 | elifesciences.org | Byrd AL |Katherine Little |Benjamin P. Bratton |Ellen M Acosta

While our human skin microbiome FISH and PMA studies strongly agree, we are unable to directly compare the two approaches on the same samples. For a system in which we could perform both FISH and PMA-ddPCR on the same samples, we turned to mouse skin, which also enabled us to ask whether our findings are human-specific. We assessed the spatial distribution of bacterial cells in mouse skin tissue using the universal bacterial EUB338 FISH probe with tissue from K14-H2B-GFP mice.

Mar 22, 2023 | virologyj.biomedcentral.com | Byrd AL

Dataset acquisition and virome analysis for auxiliary metabolic genesIn addition to 42 previously sequenced subjects presented in Graham et al. [26] (SRA project PRJNA754140) (n = 642), skin viral metagenome samples from 18 study participants (n = 80) were collected and processed as described previously [26].