Benjamin P. Bratton

Jul 12, 2024 | nature.com | Meera Gupta |Eli Costa |Thao Nguyen |Thảo Nguyen |Michael Stadlmeier |Benjamin P. Bratton | +2 more

AbstractProtein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli, proteome-wide measurements remain scarce. Here, we quantify the turnover rates of ~3200 E. coli proteins under 13 conditions by combining heavy isotope labeling with complement reporter ion quantification and find that cytoplasmic proteins are recycled when nitrogen is limited.

Mar 28, 2024 | biorxiv.org | Alp M. Sunol |Benjamin P. Bratton |Morgan Delarue |Diana Valverde-Mendez

AbstractThe crowded bacterial cytoplasm is comprised of biomolecules that span several orders of magnitude in size and electrical charge. This complexity has been proposed as the source of the rich spatial organization and apparent anomalous diffusion of intracellular components, although this has not been tested directly.

Jun 30, 2023 | elifesciences.org | Byrd AL |Katherine Little |Benjamin P. Bratton |Ellen M Acosta

While our human skin microbiome FISH and PMA studies strongly agree, we are unable to directly compare the two approaches on the same samples. For a system in which we could perform both FISH and PMA-ddPCR on the same samples, we turned to mouse skin, which also enabled us to ask whether our findings are human-specific. We assessed the spatial distribution of bacterial cells in mouse skin tissue using the universal bacterial EUB338 FISH probe with tissue from K14-H2B-GFP mice.