Caitlynn Diggs

Dec 3, 2024 | biorxiv.org | Li Shen |Caitlynn Diggs |Shomita Ferdous |Neol Wolf

AbstractThe obligate intracellular bacterial pathogen, Chlamydia trachomatis (Ct), has a distinct DNA topoisomerase I (TopA) with a C-terminal domain (CTD) homologous to eukaryotic SWIB domains. Despite the lack of sequence similarity at the CTDs between C. trachomatis TopA (CtTopA) and Escherichia coli TopA (EcTopA), full-length CtTopA removed negative DNA supercoils in vitro and complemented the growth defect of E. coli topA mutant.