Carlos H. Luna-Flores

Jul 25, 2023 | onlinelibrary.wiley.com | Nanotechnology The |Alexander Wang |Carlos H. Luna-Flores |Yilun Weng

2.5.7 Differential scanning calorimetry (DSC) Phytase was dialyzed against Milli-Q water using a dialysis membrane with a cut-off molecular weight of 10 kDa (Fisher Scientific) for 20 h at 4°C (Ariza et al., 2013). The sample was diluted with Milli-Q water to make a final phytase concentration of 1 mg/mL. Milli-Q water was used as a reference for DSC. All samples and solution were degassed with stirring for at least 15 min at room temperature.