Carolina Campelo Gutiérrez

Jul 23, 2024 | digitalcommons.library.tmc.edu | Wen Bu |Chad J. Creighton |Kelsey S. Heavener |Carolina Marinovic Gutierrez |Carolina Campelo Gutiérrez

AbstractCRISPR-Cas9 has been used successfully to introduce indels in somatic cells of rodents; however, precise editing of single nucleotides has been hampered by limitations of flexibility and efficiency. Here, we report technological modifications to the CRISPR-Cas9 vector system that now allows homology-directed repair-mediated precise editing of any proto-oncogene in murine somatic tissues to generate tumor models with high flexibility and efficiency.