Wen Bu

Jan 22, 2025 | digitalcommons.library.tmc.edu | Wen Bu |Yi Li

KeywordsMice, Animals, Mammary Glands, Animal, Epithelial Cells, Gene Transfer Techniques, Oncogenes, Retroviridae, Adenoviridae, Neoplasms, Mammary Neoplasms, Experimental, Mice, TransgenicAbstractMouse mammary glands comprise ductal trees, which are lined by epithelial cells and have one opening at the tip of each nipple. The epithelial cells play a major role in mammary gland function and are the origin of most mammary tumors.

Jul 23, 2024 | digitalcommons.library.tmc.edu | Wen Bu |Chad J. Creighton |Kelsey S. Heavener |Carolina Marinovic Gutierrez |Carolina Campelo Gutiérrez

AbstractCRISPR-Cas9 has been used successfully to introduce indels in somatic cells of rodents; however, precise editing of single nucleotides has been hampered by limitations of flexibility and efficiency. Here, we report technological modifications to the CRISPR-Cas9 vector system that now allows homology-directed repair-mediated precise editing of any proto-oncogene in murine somatic tissues to generate tumor models with high flexibility and efficiency.

Apr 4, 2024 | medium.com | Wen Bu

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Mar 29, 2024 | medium.com | Wen Bu

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Mar 28, 2024 | medium.com | Wen Bu

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