Clint McCue

Oct 24, 2024 | pubs.rsc.org | Sarah Mersch |Clint McCue |Erin D. Sheets |Alexandros Aristidou

Translational Diffusion, Molecular Brightness, and Energy Transfer Analysis of mEGFP–Linker–mScarlet–I Crowding Biosensor using Fluorescence Correlation Spectroscopy Recently, we have investigated the sensitivity of an mEGFP-linker-mScarlet-I construct (GE2.3) in response to macromolecular crowding using ensemble time-resolved two-photon (2P) fluorescence measurements [Mersch et al., PCCP 26 (5), 2024] as a point of reference for developing a single-molecule approach for Förster resonance...