Erin D. Sheets

Oct 24, 2024 | pubs.rsc.org | Sarah Mersch |Clint McCue |Erin D. Sheets |Alexandros Aristidou

Translational Diffusion, Molecular Brightness, and Energy Transfer Analysis of mEGFP–Linker–mScarlet–I Crowding Biosensor using Fluorescence Correlation Spectroscopy Recently, we have investigated the sensitivity of an mEGFP-linker-mScarlet-I construct (GE2.3) in response to macromolecular crowding using ensemble time-resolved two-photon (2P) fluorescence measurements [Mersch et al., PCCP 26 (5), 2024] as a point of reference for developing a single-molecule approach for Förster resonance...

Sep 28, 2023 | pubs.rsc.org | Sarah Mersch |Sarah Bergman |Arnold J. Boersma |Erin D. Sheets

Two-Photon Excited-State Dynamics of mEGFP–Linker–mScarlet–I Crowding Biosensor in Controlled Environments Macromolecular crowding affects many cellular processes such as diffusion, biochemical reaction kinetics, protein-protein interactions, and protein folding. Mapping the heterogeneous, dynamics crowding in living cells or tissues requires genetically encoded, site-specific, crowding sensors that are compatible with quantitative, noninvasive fluorescence micro-spectroscopy.