CSIC-Universidad de Salamanca

Jul 10, 2024 | molecular-cancer.biomedcentral.com | CSIC-Universidad de Salamanca

The Rosa26-RRAS2fl/fl knock-in mouse line was generated by homologous recombination as described [19], and it contains the WT human RRAS2 sequence with an HA-tag driven by a CAG promoter, followed by an EGFP sequence downstream of an IRES sequence, and with a LoxP-flanked stop codon at the 5’ end of the construct (Rosa26-RRAS2fl/fl). Rosa26-RRAS2fl/fl mice were crossed with Cre recombinase lines to induce conditional overexpression of this construct by removing the stop codon.

Jan 16, 2024 | gut.bmj.com | Reka Toth |Paul Brindley |Javier Vaquero |CSIC-Universidad de Salamanca

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