David Adams

Oct 24, 2024 | biorxiv.org | David Adams |Michael A. Quail |Jingtao Lilue |Iraad Bonner

AbstractIt is over twenty years since the publication of the C57BL/6J mouse reference genome, which has been a key catalyst for understanding mammalian disease biology. However, the mouse reference genome still lacks telomeres and centromeres, contains 281 chromosomal sequence gaps, and only partially represents many biomedically relevant loci. We present the first T2T mouse genomes for two key inbred strains, C57BL/6J and CAST/EiJ.

May 10, 2024 | biorxiv.org | Luuk Loeff |David Adams |Christelle Chanez |Sandrine Stutzmann

AbstractSeventh pandemic Vibrio cholerae strains contain two hallmark pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryo-EM to reveal the mechanistic basis for plasmid defense by DdmDE. A cryo-EM structure of the DdmD helicase-nuclease reveals that it adopts an auto-inhibited dimeric architecture. The prokaryotic Argonaute protein DdmE uses a DNA guide to target plasmid DNA.

Apr 4, 2024 | biorxiv.org | Alexandre Lemopoulos |David Adams |Melanie Blokesch |Grazia Vizzarro

AbstractIn response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialised defence systems that are often clustered together on genomic islands. The O1 El Tor strains of Vibrio cholerae responsible for the ongoing seventh cholera pandemic (7PET) contain a characteristic set of genomic islands involved in host colonisation and disease, many of which contain defence systems.

Aug 16, 2023 | biorxiv.org | Sneha Ralli |Tariq Vira |Carla Daniela Robles-Espinoza |David Adams

AbstractIdentifying genetic susceptibility factors for complex disorders remains a challenging task. We have developed a weights-based pipeline to prioritize variants and genes in collections of small and large pedigrees where genetic heterogeneity is likely, but biological commonalities are plausible.