David Pflieger

Aug 25, 2023 | science.org | Yun Zhu |Hideaki Yamamoto |David Elser |David Pflieger

MATERIALS AND METHODSNicotiana species with their origin and associated accession numbers are summarized in table S1. Seeds of all Nicotiana species were directly germinated on soil, with the exception of N. attenuata, for which smoke-induced seed germination was established as described previously (8). For all species, glasshouse growth conditions were as described previously (8). Six- to eight-week-old elongated plants were used for all metabolomics analyses.