Dharmesh D. Bhuva

Jun 3, 2024 | biorxiv.org | Agus Salim |Dharmesh D. Bhuva |Pengyi Yang

AbstractLibrary size normalisation is necessary to enable comparisons between observations in transcriptomic datasets. Numerous methods have been developed to normalise these effects with sample and gene specific adjustments. However, in spatial transcriptomics data, normalisation is complicated by the fact that spatial region-specific library size confounds biology.