Edward H. Egelman

Dec 17, 2024 | pubs.rsc.org | Edward H. Egelman

Cryo-EM for atomic characterization of supramolecular gels While there have been great advances in the design and synthesis of supramolecular gels, their characterization methods have largely stayed the same, with electron microscopy of dried samples, or small-angle scattering and spectroscopy dominating the approaches used.

Jul 22, 2024 | onlinelibrary.wiley.com | Edward H. Egelman

Cryo-EM has supplanted X-ray crystallography as the main technique being used to determine the atomic structure of macromolecular complexes. This transformation in structural biology has been based upon a number of developments. First, it has been known for >80 years that protein structure is only maintained in a fully hydrated environment (Bernal et al., 1938; Crowfoot & Riley, 1939).

Feb 5, 2024 | nature.com | Arundhati Deshmukh |Austin Bailey |Ellen M. Sletten |Edward H. Egelman

AbstractCryo-electron microscopy has delivered a resolution revolution for biological self-assemblies, yet only a handful of structures have been solved for synthetic supramolecular materials. Particularly for chromophore supramolecular aggregates, high-resolution structures are necessary for understanding and modulating the long-range excitonic coupling. Here, we present a 3.3 Å structure of prototypical biomimetic light-harvesting nanotubes derived from an amphiphilic cyanine dye (C8S3-Cl).

Nov 27, 2023 | pnas.org | Edward H. Egelman |Juan Carlos |Thomas Perrot |Adrien Rusch

L. Craig, K. T. Forest, B. Maier, Type IV pili: Dynamics, biophysics and functional consequences. Nat. Rev. Microbiol. 17, 429–440 (2019). J. L. Berry, V. Pelicic, Exceptionally widespread nanomachines composed of type IV pilins: The prokaryotic Swiss Army knives. FEMS Microbiol. Rev. 39, 134–154 (2015). V. Pelicic, Mechanism of assembly of type 4 filaments: Everything you always wanted to know (but were afraid to ask). Microbiology (Reading) 169, 001311 (2023). K. S. Makarova, E. V. Koonin, S. V.

May 22, 2023 | nature.com | Jiaqi Guo |Fengbin Wang |Yimeng Huang |Hongjian He |Meihui Yi |Edward H. Egelman

AbstractCell spheroids bridge the discontinuity between in vitro systems and in vivo animal models. However, inducing cell spheroids by nanomaterials remains an inefficient and poorly understood process.