Edward S. Curry

Jan 28, 2024 | biorxiv.org | Edward S. Curry |Svetlana E. Sedelnikova |John Rafferty |Martyn Hulley

AbstractAll mRNA products are currently manufactured in in vitro transcription (IVT) reactions that utilize single-subunit RNA polymerase (RNAP) biocatalysts. Although it is known that discrete polymerases exhibit highly variable bioproduction phenotypes, including different relative processivity rates and impurity generation profiles, only a handful of enzymes are generally available for mRNA biosynthesis.