Eli Costa

Jul 12, 2024 | nature.com | Meera Gupta |Eli Costa |Thao Nguyen |Thảo Nguyen |Michael Stadlmeier |Benjamin P. Bratton | +2 more

AbstractProtein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli, proteome-wide measurements remain scarce. Here, we quantify the turnover rates of ~3200 E. coli proteins under 13 conditions by combining heavy isotope labeling with complement reporter ion quantification and find that cytoplasmic proteins are recycled when nitrogen is limited.