Emil Karpinski

Jul 8, 2024 | biorxiv.org | Ahmed Mahas |Emil Karpinski |George M Church

AbstractDNA plasmids are commonly used for delivering proteins and RNA in genome editing. However, their bacterial components can lead to inactivation, cell toxicity, and reduced efficiency compared to minicircle DNA (mcDNA) lacking such bacterial sequences. Existing commercial kits that recombine plasmids into mcDNA within proprietary bacterial strains are labor-intensive, yield inconsistent results, and often produce low-quality mcDNA.