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1 month ago | 
biorxiv.org | Ahmed Mahas |Raphael Ferreira |Lisa Maria Riedmayr |George M Church
AbstractProgrammable technologies that sense specific nucleic acid signatures in living cells and trigger cellular functions hold significant potential for biotechnology and medicine. Here, we developed SONAR (Sensing Of Nucleic acids using ASOs and Reverse-transcriptases), a platform that enables the detection of target DNA and RNA sequences and triggers controlled gene expression in human cells.
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Dec 9, 2024 | 
nature.com | Esha Madan |Jochen Wilhelm |Patrick Martin |Anna Gustafsson |Narsimha Mamidi |Lin Lin | +25 more
AbstractCell competition and fitness comparison between cancer and tumor microenvironment (TME) cells determine oncogenic fate. Our previous study established a role for human Flower isoforms as fitness fingerprints, where the expression of Flower Win isoforms in tumor cells leads to growth advantage over TME cells expressing Lose isoforms.
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Oct 30, 2024 | 
nature.com | Mirazul Islam |Yilin Yang |Alan Simmons |Naila Tasneem |Zhengyi Chen |Iannish D. Sadien | +9 more
AbstractTemporal ordering of cellular events offers fundamental insights into biological phenomena. Although this is traditionally achieved through continuous direct observations1,2, an alternative solution leverages irreversible genetic changes, such as naturally occurring mutations, to create indelible marks that enables retrospective temporal ordering3,4,5.
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Oct 13, 2024 | 
biorxiv.org | Songlei Liu |George M Church |Craig Hunter |Andrei Shubin
AbstractThe prevalence and significance of intercellular mRNA transport remains unknown. Direct detection of mRNA transfer between cells within an organism is challenging due to technical limitations associated with transgene encoded molecular labels and cell sorting techniques. In this study we analyzed human-to-mouse xenograft single-cell RNA sequencing data to identify mouse transcripts in recovered human cells.
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Sep 26, 2024 | 
nature.com | Braden T. Tierney |Kristina M. Babler |Bhavarth Shukla |Stephan C. Schürer |George M Church |Helena Solo-Gabriele | +1 more
AbstractWastewater is a geospatially- and temporally-linked microbial fingerprint of a given population, making it a potentially valuable tool for tracking public health across locales and time. Here, we integrate targeted and bulk RNA sequencing (N = 2238 samples) to track the viral, bacterial, and functional content over geospatially distinct areas within Miami Dade County, USA, from 2020-2022.
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Sep 12, 2024 | 00242-x&sz=64)
cell.com | George M Church
ResourceOnline nowOpen access 1Wyss Institute at Harvard University, Boston MA, USA 2The Ken & Ruth Davee Department of Neurology and Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 3Simpson Querrey Center of Neurogenetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 4Department of Genetics, Harvard Medical School, Harvard University, Cambridge, MA, USA Publication History: Received June 19, 2024; Revised August 12,...
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Jul 22, 2024 | 
nature.com | Lindsay Rutter |Henry Cope |Nathaniel J. Szewczyk |JangKeun Kim |Eliah G. Overbey |Braden T. Tierney | +4 more
AbstractCommon and rare alleles are now being annotated across millions of human genomes, and omics technologies are increasingly being used to develop health and treatment recommendations. However, these alleles have not yet been systematically characterized relative to aerospace medicine. Here, we review published alleles naturally found in human cohorts that have a likely protective effect, which is linked to decreased cancer risk and improved bone, muscular, and cardiovascular health.
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Jul 8, 2024 | 
biorxiv.org | Ahmed Mahas |Emil Karpinski |George M Church
AbstractDNA plasmids are commonly used for delivering proteins and RNA in genome editing. However, their bacterial components can lead to inactivation, cell toxicity, and reduced efficiency compared to minicircle DNA (mcDNA) lacking such bacterial sequences. Existing commercial kits that recombine plasmids into mcDNA within proprietary bacterial strains are labor-intensive, yield inconsistent results, and often produce low-quality mcDNA.
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Jun 11, 2024 | 
nature.com | Eliah G. Overbey |JangKeun Kim |Braden T. Tierney |Jiwoon Park |Deena Najjar |Remi Klotz | +30 more
AbstractSpaceflight induces molecular, cellular, and physiological shifts in astronauts and poses myriad biomedical challenges to the human body, which are becoming increasingly relevant as more humans venture into space1-6. Yet, current frameworks for aerospace medicine are nascent and lag far behind advancements in precision medicine on Earth, underscoring the need for rapid development of space medicine databases, tools, and protocols.
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Jun 11, 2024 | 
nature.com | Christopher Mason |James Green |Jordan Baechle |Mathias Basner |Susan Bailey |Joseph Borg | +34 more
AbstractThe recent acceleration of commercial, private and multi-national spaceflight has created an unprecedented level of activity in low Earth orbit, concomitant with the largest-ever number of crewed missions entering space and preparations for exploration-class (lasting longer than one year) missions. Such rapid advancement into space from many new companies, countries and space-related entities has enabled a ‘second space age’.
