Eray Enustun

2 months ago | cell.com | Yajie Gu |Huan Li |Amar Deep |Eray Enustun |Dapeng Zhang |Kevin Corbett

Keywords anti-phage immunity DUF4263 PD-(D/E)XK nuclease Whirly domain two-component signalling Introduction The ongoing evolutionary conflict between bacteria and their viral pathogens (bacteriophages or phages) has driven the development of myriad bacterial immune systems that fight infection and an equally complex array of viral factors that counter these systems.1,2 The most well-understood bacterial immune systems, restriction-modification systems and CRISPR-Cas systems, are found in a...

Aug 10, 2023 | biorxiv.org | Yajie Gu |Huan Li |Amar Deep |Eray Enustun

AbstractProkaryotes encode diverse anti-bacteriophage immune systems, including the single-protein Shedu nuclease. Here we reveal the structural basis for activation of Bacillus cereus Shedu. In the inactive homotetramer, a key catalytic residue in Shedu's nuclease domain is sequestered away from the catalytic site. Activation involves a conformational change that completes the active site and promotes assembly of a homo-octamer for coordinated double-strand DNA cleavage.

May 18, 2023 | biorxiv.org | Amar Deep |Yajie Gu |Katrina Nguyen |Eray Enustun

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