Feng Guo

Dec 31, 2024 | mdpi.com | Feng Guo

1. IntroductionWith the rapid development of modern transportation systems, the application of onboard equipment in vehicle management, toll collection, and monitoring is becoming increasingly widespread. Particularly in the electronic toll collection (ETC) system [1], the health status of onboard equipment directly impacts the reliability and efficiency of the system. Therefore, conducting real-time data analysis and health status evaluation of onboard equipment is of utmost importance [2,3,4].

Dec 3, 2024 | mdpi.com | Feng Guo |Aiyan Xu |Yuchen Zhang |Xuancen Li

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Oct 20, 2024 | nature.com | Minjia Zheng |Xinlan Chen |Hao LI |Wei Zheng |Feng Guo |Laiting Si | +3 more

The low cultivability of environmental bacteria has been widely acknowledged, but most previous estimates focused on the proportion of cultivable cells rather than cultivable taxa. Here, we estimate the proportions of cultivable cells and cultivable taxa for two sample types (soil and activated sludge) using cell counting, 16S rRNA gene amplicon sequencing, metagenomics, and cultivation on agar plates under various conditions. We find that the proportion of cultivable taxa exceeds that of cultivable cells at the sample level. A large proportion of cultivable taxa are taxonomically novel but tend to be present at very low abundance on agar plates, forming microcolonies, and some of them cease to grow during subculture. Compared with uncultivable taxa (under the conditions used in our study), cultivatable taxa tend to display higher metabolic activity as inferred by measuring rRNA copies per cell. Finally, we use the generated taxonomic and genomic information as a guide to isolate a strain representing a yet-uncultured class within the Bacteroidota and to enhance the cultivable diversity of Burkholderiales from activated sludge. The low cultivability of most environmental bacteria is well known, but previous estimates focused on the proportion of cultivable cells. Here, the authors use various techniques to show, for soil and activated sludge samples, that the proportion of cultivable taxa exceeds that of cultivable cells, and identify genetic and physiological traits associated with cultivability.

Sep 30, 2024 | mdpi.com | Yaqi Zhang |Feng Guo |Huan Zhang |Mingming Zhang

All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess.

Apr 7, 2024 | mdpi.com | Feng Guo |Anna Hu |Huan Zhou |Hui Hu

All articles published by MDPI are made immediately available worldwide under an open access license. No specialpermission is required to reuse all or part of the article published by MDPI, including figures and tables. Forarticles published under an open access Creative Common CC BY license, any part of the article may be reused withoutpermission provided that the original article is clearly cited. For more information, please refer tohttps://www.mdpi.com/openaccess.