Filip Boskovic

Aug 17, 2024 | biorxiv.org | Filip Boskovic |Sarah Sandler |Yuan Shui |Simon Brauburger

AbstractEffective treatment of infectious diseases hinges on timely and accurate diagnosis. Current methods face challenges in distinguishing between pathogens that cause a similar symptom and where appropriate treatment may be hindered by antimicrobial resistance (AMR). Here, we introduce a PCR-free system that identifies and quantifies ribosomal RNA transcripts (rRNA) in pathogenic bacteria instead of DNA-encoding rRNA genes.

Feb 24, 2024 | nature.com | Filip Boskovic

AbstractTranscription, a critical process in molecular biology, has found many applications in RNA synthesis, including mRNA vaccines and RNA therapeutics. However, current RNA characterization technologies suffer from amplification and enzymatic biases that lead to loss of native information. Here, we introduce a strategy to quantitatively study both transcription and RNA polymerase behaviour by sizing RNA with RNA nanotechnology and nanopores.