Frank W. Pun

2 weeks ago | advanced.onlinelibrary.wiley.com | Christine Lim |Yuqi Bian |Alicia Diaz |Alicia Díaz |Frank W. Pun

1 Introduction Membraneless organelles (MLOs) are macromolecular assemblies that lack lipid membranes but are crucial for orchestrating key cellular processes such as gene regulation, RNA metabolism, and response to stress.[1-5] Given their central roles in managing cellular homeostasis, the improper assembly and disassembly of MLOs may impair the optimal functioning of the cell, contributing to pathological phenomena.[4-7] For example, the dysregulated assembly of stress granules has been...

Nov 15, 2024 | biorxiv.org | Christine Lim |Yuqi Bian |Alicia Gonzalez-Diazag |Frank W. Pun

AbstractMembraneless organelles (MLOs) are dynamic macromolecular condensates that act as crucibles to modulate biological process within the cell. Since MLOs form in the absence of lipid membranes, it is important to understand how their effective regulation is achieved by the protein homeostasis (proteostasis) system. To address this question, we report a comprehensive mapping of the proteostasis network (PN) of MLOs, comprising over 220,000 protein-protein interactions.