Franz Oswald

2 months ago | nature.com | Tobias Friedrich |Marek Bartkuhn |Benedetto Daniele Giaimo |Tilman Borggrefe |Franz Oswald

AbstractTranscription factors (TFs) such as RBPJ in Notch signaling bind to specific DNA sequences to regulate transcription. How TF-DNA binding kinetics and cofactor interactions modulate gene regulation is mostly unknown. We determine the binding kinetics, transcriptional activity, and genome-wide chromatin occupation of RBPJ and mutant variants by live-cell single-molecule tracking, reporter assays, and ChIP-Seq.

Jul 24, 2024 | medrxiv.org | Sarah Merz |Anne Philippi |Franz Oswald |Valerie Senee

The authors have declared no competing interest. S.M. and S.H. are supported by DFG grant HE 7277/3-1 and the Medical Scientist Program at Ulm University awarded to S.H. This project was also funded by an ANR-DFG collaborative research project (grant no.