Marek Bartkuhn

2 months ago | nature.com | Tobias Friedrich |Marek Bartkuhn |Benedetto Daniele Giaimo |Tilman Borggrefe |Franz Oswald

AbstractTranscription factors (TFs) such as RBPJ in Notch signaling bind to specific DNA sequences to regulate transcription. How TF-DNA binding kinetics and cofactor interactions modulate gene regulation is mostly unknown. We determine the binding kinetics, transcriptional activity, and genome-wide chromatin occupation of RBPJ and mutant variants by live-cell single-molecule tracking, reporter assays, and ChIP-Seq.

Jan 7, 2025 | nature.com | Julianne Funk |Pascal Hunold |Dimitrios-Ilias Balourdas |Marek Bartkuhn |Rajkumar Savai |Thierry Soussi | +2 more

AbstractThe mutational landscape of TP53, a tumor suppressor mutated in about half of all cancers, includes over 2,000 known missense mutations. To fully leverage TP53 mutation status for personalized medicine, a thorough understanding of the functional diversity of these mutations is essential. We conducted a deep mutational scan using saturation genome editing with CRISPR-mediated homology-directed repair to engineer 9,225 TP53 variants in cancer cells.

Dec 9, 2024 | jbc.org | Technion City |Daniela Fietz |Monika Fijak |Marek Bartkuhn

KeywordstestisspermatogenesisIRP1iron homeostasisreactive oxygen species (ROS)DNA damageDNA repairIntroductionInfertility is a multifaceted condition, affecting approximately one in seven couples in Western countries, with the male factors contributing to nearly 50% of cases, figures projected to increase (1–3).

Dec 7, 2023 | biorxiv.org | Benedetto Daniele Giaimo |Tobias Friedrich |Francesca Ferrante |Marek Bartkuhn

AbstractTranscriptional specificity is often determined by transcription factor levels and/or chromatin context. In the Notch signal transduction pathway, transcription factor RBPJ is the central component and directly forms a coactivator complex together with the Notch intracellular domain (NICD). While the RBPJ protein levels remain constant in most tissues, dynamic expression of Notch target genes varies depending on the given cell-type and the Notch activity state.