Grace Hibshman

Nov 19, 2024 | biorxiv.org | Grace Hibshman |David Taylor

AbstractThe Cas9 endonuclease from Streptococcus pyogenes (SpCas9) has revolutionized genome editing, as it can be programmed by an RNA sequence to target and cleave DNA. However, SpCas9 is prone to deleterious off-target effects, prompting efforts to develop and discover high-fidelity variants. In the search for a more precise genome editor, many intrinsically specific Cas9 orthologs have been found in nature.

Oct 24, 2024 | biorxiv.org | Grace Hibshman |David Taylor

AbstractThe constant threat of foreign genetic elements destroying bacteria has driven the evolution of diverse defense mechanisms including clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) systems. The Cas9 endonuclease from Streptococcus pyogenes (SpCas9) has revolutionized genome editing, as it can be programmed by an RNA sequence to target and cleave DNA.