Haiyan Lu

2 months ago | nature.com | Hua Zhang |Lang Ding |Amy Hu |Xudong Shi |Penghsuan Huang |Haiyan Lu | +3 more

The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput in situ mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here we developed a tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass-spectrometry imaging (TEMI). TEMI reaches single-cell spatial resolution without sacrificing voxel throughput and enables the profiling of hundreds of biomolecules, including lipids, metabolites, peptides (proteins), and N-glycans. Using TEMI, we mapped the spatial distribution of biomolecules across various mammalian tissues and uncovered metabolic heterogeneity in tumors. TEMI can be easily adapted and broadly applied in biological and medical research, to advance spatial multi-omics profiling. Tissue-expansion method compatible with mass-spectrometry imaging (TEMI) enables the profiling of lipids, metabolites, peptides, proteins, and N-glycans in complex tissues with high spatial resolution, advancing spatially resolved multi-omics mapping.

May 13, 2024 | link.aps.org | Li Huang |Haiyan Lu

The high-temperature δ phase of plutonium can be stabilized at room temperature by doping it with a few-percent gallium, and the cubic phase Pu3Ga plays a crucial role in understanding the mechanism of the stabilized δ phase of plutonium-gallium alloy.