Haoqing Wang

Jun 28, 2024 | nature.com | Haoqing Wang |Qian Su

AbstractThe microgeometry of the cellular microenvironment profoundly impacts cellular behaviors, yet the link between it and the ubiquitously expressed mechanosensitive ion channel PIEZO1 remains unclear. Herein, we describe a fluorescent micropipette aspiration assay that allows for simultaneous visualization of intracellular calcium dynamics and cytoskeletal architecture in real-time, under varied micropipette geometries.

Apr 12, 2024 | biorxiv.org | Marina Casiraghi |Haoqing Wang |Patrick Brennan |Chris Habrian

AbstractG protein coupled receptors (GPCRs) exhibit varying degrees of selectivity for different G protein isoforms. Despite the abundant structures of GPCR-G protein complexes, little is known about the mechanism of G protein coupling specificity. The β2-adrenergic receptor is an example of GPCR with high selectivity for Gαs, the stimulatory G protein for adenylyl cyclase, and much weaker for the Gαi family of G proteins inhibiting adenylyl cyclase.

Apr 10, 2024 | biorxiv.org | Marina Casiraghi |Haoqing Wang |Patrick Brennan |Chris Habrian

AbstractG protein coupled receptors (GPCRs) exhibit varying degrees of selectivity for different G protein isoforms. Despite the abundant structures of GPCR-G protein complexes, little is known about the mechanism of G protein coupling specificity. The β2-adrenergic receptor is an example of GPCR with high selectivity for Gαs, the stimulatory G protein for adenylyl cyclase, and much weaker for the Gαi family of G proteins inhibiting adenylyl cyclase.

Mar 29, 2024 | biorxiv.org | Marina Casiraghi |Haoqing Wang |Patrick Brennan |Chris Habrian

AbstractG protein coupled receptors (GPCRs) activated by their native hormone or neurotransmitter exhibit varying degrees of selectivity for different G protein isoforms. Despite the abundant structures of different GPCR-G protein complexes, little is known about the mechanism of G protein coupling specificity.

Dec 7, 2023 | mdpi.com | Haoqing Wang |Yuan Liu |Ying Yang |Ran Yan

1. IntroductionMaritime transportation is the backbone of the global trade and manufacturing supply chain [1]. According to the report of the United Nations Conference on Trade and Development, more than 80% of world merchandise trade by volume is carried by sea in 2021 [1].