Henan Provincal Engineering

Aug 22, 2023 | bmcvetres.biomedcentral.com | Henan Provincal Engineering |Nanyang Normal

To prepare the standard positive control, the whole genome of PCV4-LY2020 (Accession no. MW759026) was synthesized and cloned into pBluescript II SK (+) vector. The recombinant plasmid named pSK-PCV4 was transformed into in E. coli DH5α and subsequently purified with a Plasmid MiniPrep Kit (OMEGA Biotech, Shanghai, China). The DNA concentration of the plasmid construct pSK-PCV4 was quantified using the NanoDrop 2000 spectrophotometer (Thermo Fisher, Waltham, MA, USA).