Hermie Harmsen

Nov 8, 2023 | nature.com | Johanne E Spreckels |Hermie Harmsen |Jingyuan Fu

AbstractHuman milk microbiome studies are currently hindered by low milk bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method to study milk bacteria and assess bacterial sharing between maternal and infant microbiota. We tested four DNA isolation methods, two bacterial enrichment methods and three sequencing methods on mock communities, milk samples and negative controls.